The Correlation of Limousin Bulls Fresh Semen Quality and Their Genetic Trait according to Polymorphic Secreted Phosphoprotein 1 (SPP1) and Follicle Stimulating Hormone Receptor (FSH-R) Encoding Gene Restriction Analysis


Yudit Oktanella , Astika D. S. Setiyoningrum , Syafirah Fauziah Rianto , Wawid Purwatiningsih , Andreas Bandang Hardian , Dyah Kinasih Wuragil , Aulanni ,

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Volume 4 - June 2020 (06)


This research aimed to elaborate the presence of polymorphisms in Secreted Phosphoprotein 1 (SPP1) and Follicle Stimulating Hormone Receptor (FSH-R) encoding genes using Polymorphism Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) which were then correlated to sperm concentration and motility percentage in bulls. A total of twenty samples of bull whole blood and their fresh semen were collected. The semen samples were evaluated to screen the sperm motility and concentration in standard manners. We performed DNA extractions from the bulls’ whole blood followed by amplification of SPP1 and FSH-R encoding gene and enzymatic digestion using HindIII. The number of bands after enzymatic restriction and the results of semen quality examination were analyzed using the Spearman test (α=0.05). The number of cleaved SPP1 gene fragments and sperm concentration showed a weak positive correlation (r=0.231) in contrary with the correlation to the semen motility percentage which showed a very weak negative value (r=-0.0728). The number of cleaved FSH-R gene fragments towards sperm concentration and motility percentage were correlated which respectively showed a very weak negative (r=-0.0364) and a very weak positive correlation (r=0.0840). Hence, there’s no significant correlation of SPP1 and FSH-R encoding gene polymorphisms towards bull sperm concentration and motility percentage (p>0.05).


FSH-R gene, Limousin bull, PCR RFLP, semen quality, SPP1 gene


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