Propagation of Leatherleaf Fern ( Rumohra adiantiformis) from Rhizomes by In vitro Techniques

Author(s)

D.L.C.Kumari Fonseka ,

Download Full PDF Pages: 113-119 | Views: 187 | Downloads: 67 | DOI: 10.5281/zenodo.3986621

Volume 4 - April 2020 (04)

Abstract

Among Floricultural plants, ornamental foliage plants have gained preference over flowering plants because; those plants can be used for a longer period of survival indoors. From them, ferns play a major role in the wholesale and retail florist business because of its popularity as ornamental plants and cut fronds. One of the most attractive ferns is the “leatherleaf fern” of family Dryopteridaceae. A novel micropropagation protocol for leatherleaf fern (Rumohra adiantiformis (G. Forst.) Ching) was successfully established using rhizomes as the explant. Murashige and Skoog (MS) medium containing 0.5 mg/L Kinetin (KIN), 1.0 mg/L 6-Benzylaminopurine (BAP) and 0.1 mg/L,  1- Naphthalene acetic acid (NAA) with 30 g/l sucrose was the most appropriate medium for culture initiation with the highest number of fronds (36) and rhizomes (20). R. adiantiformis cultures could be successfully proliferated using full-strength MS medium supplemented with 0.1 mg/L BAP, 0.1 mg/L NAA, 1.0 mg/L KIN and 0.1 mg/L BAP, 0.2 mg/l NAA, 1.0 mg/l KIN. Both hormonal combinations showed the highest proliferation rate (6.28) of regenerated rhizomes with no significant difference at P≤0.05. For commercial scale propagation of leather leaf fern, the treatment which contained 0.1 mg/l BAP, 0.1 mg/l NAA, 1.0 mg/l KIN was selected as the most suitable medium and the efficiency of the medium was observable from the first subculture through to the fourth subculture. The level of multiplication peaked in the fifth subculture and retained high quality until the sixth subculture. From the seventh Rumohra adiantiformis subculture onwards, the quality of regenerated fronds was reduced. Effective rooting was undertaken on 50% MS basal medium + glucose (15 g/L) + Ascorbic acid (100 mg/ L) and gelled with 0.8% (w/v) agar supplemented with NAA 1 mg/L with highest number of roots (6-8), 12-15 days after inoculation. All the acclimatized plantlets showed 100% survival percentage after 30 days.

Keywords

In vitro propagation, Commercial scale, Leatherleaf fern, Rhizome culture

References

                   i.            Avila-Pérez, M.D.C.R., White-Olascoaga, L. & Arzate-Fernández, A.M., 2011. In Vitro Regeneration of Leatherleaf Fern (Rumohra adiantiformis (G.Forst.) Ching). American Fern Journal, 101(1),25–35. Available at: http://www.bioone.org/doi/abs/10.1640/0002-8444-101.1.25.

      ii.            Brum, F.M.R., Randi, A.M., 2006. Germination of spores and growth of gametophytes and sporophytes of Rumohra adiantiformis (Forst.) Ching (Dryopteridaceae) after spore cryogenic storage. Rev. Bras. Bot. 29, 489–495.

    iii.            Chen, S.Y., Read, P.E., 1983. Micropropagation of leatherleaf fern (Rumohra adiantiformis).Proc. Fla. State Hortic. Soc. 96, 266–269.

     iv.            D’Souza, G.C., Kubo, R., Guimarães, L., Elisabetsky, E., 2006. An ethnobiological assessment of Rumohra adiantiformis (samambaia-preta) extractivism in Southern Brazil. Biodivers. Conserv. 15, 2737–2746.

       v.            Freshney, R.I., 2006. Basic principles of cell culture. In: Vunjak-Novakovic, G., Freshney,R.I. (Eds.), Culture of Cells for Tissue Engineering. John Wiley & Sons, Inc., London, pp. 4–22.

     vi.            Hamad, A.M., Taha, R.M., 2008. Effect of sequential subcultures on in vitro proliferation capacity and shoot formations pattern of pineapple (Ananas comosus L. Merr.) over different incubation periods. Sci. Hortic. 117 (4), 329–334.

   vii.            Karuppusamy, S., Kiranmai, C., Aruna, V., Pullaiah, T., 2009. In vitro conservation of Ceropegia intermedia—an endemic plant of south India. Afr. J. Biotechnol. 8 (17), 4052–4057.

 viii.            Khaleghi, A., Khalighi, A., Sahraroo, A., Karimi, M., Rasoulnia, A., Ghafoori, I.N., Ataei, R., 2008. In vitro propagation of Alstroemeria cv. ‘Fuego’. American-Eurasian J. Agric. Environ. Sci. 3 (3), 492–497.

     ix.            Raihana, R., Faridah, Q.Z., Julia, A.A., Abdelmageed, A.H.A., Kadir, M.A., 2011. In vitro culture of Curcuma mangga from rhizome bud. J. Med. Plants Res. 5 (28), 6418–6422.

       x.            Rani, A.S., Subhadra, V.V., Reddy, V.D., 2000. In vitro propagation of Acorus calamus Linn.—a medicinal plant. Indian J. Exp. Biol. 38 (7), 730–732.

     xi.            Reid, M.S., 2004. Leatherleaf Fern: Recommendations for Maintaining Postharvest Quality. Postharvest Technology Research & Information Center, University of California, Davis.

   xii.            Strandberg JO (2003) Seasonal variations in production and development of leatherleaf fern leaves. Annals of Applied Biology 143, 235-243

 xiii.            Sunitibala, H., Damayanti, M., Sharma, G.J., 2001. In vitro propagation and rhizome formation in Curcuma longa Linn. Cytobios 105 (409), 71–82.

 xiv.            Swapna, T.S., Binitha, M., Manju, T.S., 2004. In vitro multiplication in Kaempferia galanga Linn. Appl. Biochem. Biotechnol. 118 (1–3), 233–241.

   xv.            Yousef, H., Sahar, B., Abdollah, H., 2007. In vitro propagation of Alstroemeria using rhizome explants derived in vitro and in pot plants. Afr. J. Biotech. 6 (18), 2147–2149.

 xvi.            Winarto, B. & Teixeira da Silva, J.A., 2012. Improved micropropagation protocol for leatherleaf fern (Rumohra adiantiformis) using rhizomes as donor explant. Scientia Horticulturae, 140(February), pp.74–80. Available at: http://dx.doi.org/10.1016/j.scienta.2012.03.017.

xvii.            Winarto, B. & Teixeira, J.A., 2012. Sterilization Procedure for in Vitro Culture of Leatherleaf Fern ( Rumohra adiantiformis ). International Journal of Plant Developmental Biology, 6(1), pp.46–50.

xviii.            Zhao, D.L., Wang, X.Y., Lu, W., Zheng, G.C., 2003. Plant regeneration via organogenesis from adventitious bud explants of a medicinal herb species, Polygonatumcyrtonema. In Vitro Cell. Dev. Biol.—Plant. 39 (1), 24–27.

 xix.            Zhao, Q., Wu, C., Wang, W., Yuan, S., Bao, J., Chen, F., 2009. In vitro plantlet regeneration system from rhizomes and mannose-binding lectin analysis of Polygonatum cyrtonema Hua. Plant Cell Tiss. Organ Cult. 99 (3), 269–275.

Cite this Article: