Making Probiotic Trichoderma sp. Using Rice Bran Media with Addition of Molasses
Probiotics are one of the products that are beneficial for livestock. Giving probiotics to livestock can improve their metabolic processes. Trichoderma is one of the microbiology that has antagonistic properties, namely suppressing the presence of other microbiology (pathogens). Therefore the researchers wanted to know the effect of giving molasses to the growth of Trichoderma sp in probiotics.This study aims to determine the effect of adding molasses to the growth of Trichoderma sp. in making probiotics using rice bran media. This study used a completely randomized design method (CRD) using four treatments and six replications. The treatment used is P0 = Rice Bran + 1% Trichoderma sp., P1 = Rice Bran + 1% Trichoderma sp. + 1% Molasses, P2 = Rice Bran + 1% Trichoderma sp. + 3% Molasses, P3 = Rice Bran + 1% Trichoderma sp. + 5% Molasses. Giving Molasses in the manufacture of probiotics on rice bran media had a significant effect (P> 0.05) on the growth of Trichoderma sp. but did not provide a significant difference in the decrease in pH (P <0.05). with the best results on P2 with 5.22 x 1010/g media spores
Trichoderma sp, pH, molasses, rice bran media, probiotic
i. Alfizal, Marlina and Susanti, F. 2013. The Ability of Antagonist Trichoderma sp. Against Some Pathogenic Fungus In Vitro.. Journal Floratek, vol 8, hal 45 -51.
ii. Amaria, W., Soesanthy, F. and Ferry, Y. 2016. The Effectiveness Of Biofungicide Trichoderma sp. With Three Kinds Of Carrier On White Root Disease Rigidoporus microporus. Journal of Industrial Plants and Fresheners, vol 3 (1), hal 37-44.
iii. Dhama, K., Mahendran, M., Tomar, S. And Chauhan, R. S. Without Years. Beneficial Effects of Probiotics and Prebiotics in Livestock and Poultry: The Current Perspectives. Department of Animal Sciences, Central Agricultural Research Institute (CARI), Port Blair, A&N Islands - 744 101, hal 1-12.
iv. Fifendy, M., Eldini and Indrawati. 2013. The Effect of Molasses Using the Number of Microbes And Nata Thickness in Kombucha Tea. Semirata Proceedings of FMIPA University of Lampung, hal 67-72.
v. Fuller, R. 1989. Probiotics in Man and Animals. Journal of Applied Bacteriology, vol 66, hal 365-378
vi. Gusnawati, H. S., Taufik, M., Bande, O. S. and Asis, A. 2017. Effectiveness of Several Media for Propagation Biological Agent Trichoderma sp. Journal HPT Tropika, vol 16, hal 70-76.
vii. Harman, G. E., Howel, C. R., Viterbo, A., Chet, I. and lorito, M. 2004. Trichoderma Species Opportunistic,Avirulent Plant Symbionts. Nature Reviews Microbiology, vol 2, hal 43-55.
viii. Ismi, R. S., Pujianingsih,R. I. and Sumarsih, S. 2017. The Effect of Molases Level Addition on Physical and Organoleptic Quality of Goat Feed Pellets on Fattening Period. Journal Integrated Scientific Animal Husbandry, vol 5 (3), hal 58-62.
ix. Juliana, Umrah and Asrul. 2017. Miselium Growth Trichoderma Sp. on Tempe Liquid Waste and Coconut Waste. Journal Biocelebes, vol 12 (2), hal 52-59.
x. Kusmiati, Tamat, S. R., Jusuf, E. and Istiningsih, R. 2007. Beta Glucan Production from Two Strains of Agrobacterium sp in Medium Containing of Molases and Uracil Combine. Journal Biodiversitas, vol 8 (1), hal 123-129.
xi. Nainggolan, J. 2009. Study of The Growth of The Bacterium Acetobacter sp. in The Red Kombucha (Hibiscus sabdariffa) at Different Sugar Levels and Fermentation Times. Thesis. Biologi Program. North Sumatra University. North Sumatra.
xii. Susatyo, J. H. 2016. Differences Influence Basting and Immersion Alcohol 70% Decrease Score Against Germs Count on Tools Dentistry. Health Vocational Journal, vol 2 (2), hal 160-164.
xiii. Uruiral, C., Kalay, A. M., Kaya, E. and Siregar, A. 2012. Utilization of Sago Waste, Rice Husk and Bran as Media for Multiplication of The Biological Agents Trichoderma harzianum Rifai. Journal Agrologia, vol 1, hal 21-30.
xiv. Wahyudi, P., Suwahyono, U. and Mulyati, S. 2004. Growth of Trichoderma harzianum in Medium Containing Xilan. Journal Indonesian Pharmaceutical Science, vol 2, 31-36.
xv. Yuningsih, R. 2007. Antibacterial Activity from Extract of Jawer Kotok (Coleus scutellaroides [L] Benth.) Leaves. Thesis. Biochemistry Program. Bogor Institute of Agriculture.Cite this Article: