An Efficient Protocoal for Isolation of Genomic DNA form Leaf Tissues of Mulberry Plant (Morus spp.) Using CTAB Method
Isolation of genomic DNA is the first and foremost step in any type of molecular study. The purity of DNA is very crucial for success of genomic studies. Since mulberry (Morus spp.) have high phenolic content, it becomes difficult to isolate fairly pure DNA which hinders the results of molecular studies. In present experiment an attempt has been made to develop an efficient and cost effective protocol for isolation of DNA from mulberry leaves. The quality of DNA was observed on gel electrophoresis, and purity of DNA as revealed on spectrophotometer by ratios of absorbance at 260/280nm was closer to 2.0, which indicated the presence of fairly pure DNA. Genomic DNA was analysed for PCR amplification with SSR markers further confirmed its purity
Mulberry, DNA, protocoal, electrophoresis, spectrophotometer, SSR markers
i. Allen. G.C., Flores-Vergara. M.A., Krasnyanski. S., Kumar. S. and Thompson. W.F. 2007. A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide. Nature Protocols. 1(5) 2321.
ii. Anuradh H. J., Vijayan K., Nair C.V. and Manjula,. A. 2013. A novel and efficient protocol for the isolation of genomic DNA from mulberry (Morus L.). Emirates Journal of Food and Agriculture .25 (2): 124-131.
iii. Arruda. S.R., Pereira. D.G., Silva-Castro. M.M., Brito. M.G and Waldschmidt. A.M. 2017. An optimized protocol for DNA extraction in plants with a high content of secondary metabolites, based on leaves of Mimosa tenuiflora (Willd.) Poir. (Leguminosae). Genetics and Molecular Research. 16 (3): gmr16039063.
iv. Doyle, J. J. and Doyle, J. L. 1990. Isolation of plant DNA from fresh tissue. Focus, 12: 13-15.
v. Tiwari. S., Tomar. R. S., Tripathi. M. K. and Ahuja. A. 2017. Modified Protocol for Plant Genomic DNA Isolation. Indian Research. Journal of Genetic and Biotechnology. 9(4) 478 – 485.
vi. Youssef. M.,Valdez-Ojeda. R., Ku-Cauich. J. R. and Medrano. R. M. E. 2015. Enhanced Protocol for Isolation of Plant Genomic DNA. Journal of Agriculture and Environmental Sciences. 4 (2): 172-180.Cite this Article: